Asian Journal of Research in Chemistry

M. D. Wandhare ¹, U. A. Deokate ¹, S. S. Khadabadi ¹, S. P. Hadke ¹, H. A. Sawarkar ¹

Affiliations
1 Dept. of Pharmacognosy and Phytochemistry, Government College of Pharmacy, Kathora Naka, Amravati-440064 (M.S.), IN

Abstract

A rapid and sensitive high-performance thin-layer chromatographic (HPLC) method was developed and validated for the quantitative estimation of Curcumin in formulation containing Curcuma longa extracts. Simple extraction method was used for isolation of curcumin from formulation sample. The isolated samples were chromatographed by using RP-HPLC Method on HiQ Sil C18 W 4.6 mm×250 mm i.d. column and detected by UV at 420 nm. The linearity range for Curcumin was 0.4-2.4 mcg/ml with average recovery of 97.57±0.5137. The limit of detection and limit of quantification was found to be 3.0196 and 9.1503 for curcumin respectively.The developed method was successfully applied for the assay of market formulations containing Curcuma longa extracts.

Keywords

RP-HPLC, Curcuma Longa Extracts, Curcumin.

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Trapti Rastogi ¹, Vijay Bhutda ¹, Komal Moon ¹, P. B. Aswar ¹, S. S. Khadabadi ¹

Affiliations
1 Government College of Pharmacy, Kathora Naka Road, Amravati-444604, IN

Abstract

Ethanolic extracts of Moringa oleifera and Vitex negundo were taken for anthelmintic activity against Indian earthworm Pheritima posthuma. Various concentrations of both extracts were tested and results were expressed in terms of time for paralysis and time for death of worms. Piperazine citrate (10 mg/ml) was used as a reference standard and distilled water as a control group. Dose dependent activity was observed in both plant extracts but Moringa oleifera shows more activity as compared to Vitex negundo.

Keywords

Moringa oleifera, Vitex negundo, Anthelmintic activity, Piperazine Citrate.

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V. B. Kshirsagar ¹, U. A. Deokate ¹, V. B. Bharkad ¹, S. S. Khadabadi ¹

Affiliations
1 Government College of Pharmacy, Amravati-444604 (M.S.), IN

Abstract

The stationary phase silica gel G60F254 was selected for separation and the sample was developed using a mixture of Toluene:Ethyl acetate:Formic acid:GAA in the ratio 2:1:1:0.75 v/v as mobile phase. Quantification was carried out at 194 nm for Diosgenin and 280 nm for Levodopa using absorbance reflectance mode. The Rf value of Levodopa and Diosgenin was found to be 0.27+0.2 and 0.61+0.2 respectively. Linearity was found to be in the concentration range of 100 to 700 ng/spot of Levodopa and 600 to 1800 for Diosgenin the correlation coefficient value is 0.9954 and 0.9934. The results of analysis were validated in terms of accuracy and precision. The LOD was found to be 1.03 ng and 5.69 ng of Levodopa and Diosgenin respectively. LOQ was found to be 3.14 ng and 17.25 ng/spot of Levodopa and Diosgenin respectively. The content uniformity test was carried out as per the USP specification. The proposed HPTLC method provides a faster and cost effective quantitative control for routine analysis of Levodopa and Diosgenin.

Keywords

Simultaneous Estimation, Levodopa, Diosgenin, Validation.

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